Reconstitution Basics

Definition

Reconstitution describes the controlled introduction of a sterile solvent — typically bacteriostatic water — into a lyophilised peptide vial, producing a stable solution suitable for research observation.

Process Principles

• Sterile environment
  Maintain aseptic technique throughout.
• Slow introduction
  Allow solvent to run down vial wall, not directly onto powder.
• Gentle dissolution
  Swirl rather than shake to preserve peptide bonds.
• Refrigerate
  Store the resulting solution at 2–8°C immediately.